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确定AlbuminandIgGDepletionFromSerum/PlasmaforProteomics
IgGremoval 90%(70-80%oftotalImmunoglobulinsremoved)Albuminremoval 95%Seamlessandsimple 1hourprotocolLowabundanceenrichmentequivalenttoimmuno-affinityDisposable,cost-effective,nocolumnregenerationorcross-contaminationWorksformostspeciestestedincludinghuman,sheep,rat,mouse,bovineOn-beadprotocolsimproveworkflowandefficiency,especiallysuitedtotargetedproteomicsSuitableforLC-MS,1and2DGels,ELISAs,EnzymeandotherFunctionalAssays.Theclassicalplasmaproteinsgenerallyfallintofunctionalcategories,formingthevastmajorityofthemid-to-highabundanceproteome.Inserum,thesesub-proteomesbymasscontentare:Albumin50-60%;Immunoglobulins10-20%;TransportProteins(Transferrin,Apo)5-10%;ComplementrelatedProteins3-5%;ProteaseInhibitors2-5%;andallothers2-5%.Whilethesesub-proteomesarerequiredfornormalbodyhomeostasis,theyneverthelesscanbecomedysfunctionalduringacute-phaseandchronicstimuli.
So,dependingontheneedsoftheinvestigation,itcanbevaluabletoconsiderthatoneormoreofthesecategoricalsub-proteomesissimplybackgroundnoisewherebydepletionisbeneficial.Whileinothercases,thesesamecategoricalsub-proteomesmightprovidenewdataandinformationandconsequently,shouldnotbedepleted.DifferentAlbuVoid™,AlbuVoid™PLUSandAlbuSorb™PLUSworkflowssupportdifferentproteomicbiasesasoutlinedinthefollowingTable.
Productsanddigestconditionsproducedifferentsub-proteomewindowsofobservation.So,dependingontheneedsoftheinvestigation,itcanbevaluabletoconsiderthatoneormoreofthesecategoricalsub-proteomesissimplybackgroundnoisewherebydepletionisbeneficial.Whileinothercases,thesesamecategoricalsub-proteomesmightprovidenewdataandinformationandconsequently,shouldnotbedepleted.Categoricallytheacute-phasesub-proteomesdifferentiatedindiseasemayvarygreatlyfromthoseassociatedwithchronicsub-proteomes.Sothereisgreatbenefitinhavingoptionstoenrichordepleteoneormoreofthesesub-proteomes.
BSG\"sAlbuminandIgGRemovalKitsoffermanysuchoptions:
The\"PLUS\"productssubstantiallydepleteImmunoglobulinsthroughseparationsattheproteinlevel.ThevariableregionsofImmunoglobulinsareextremelyheterogeneous,generatingabackgroundnoiseacrossthefullLCgrADIent.On-beaddigestion(BASP™)withAlbuVoid™substantiallyreducestheinfluenceofsuchIgpeptidefeatures.Soinadditiontoworkflowsimplicity,BASP™canbeadvantageousutilizedintargetedproteomicworkflowswheneverthetargetproteinsdonotrequirestrongdenaturingconditions.WiththeexceptionofImmunoglobulinswherebyFASPgeneratesmanymorespectralfeatures,bothstrongdenaturingconditions(FASP)andon-beaddigest(BASP™)conditionsproducesimilarproteinprofiles.BothApolipoproteinsandheavilyglycosylatedproteins(i.e.,α1-AcidGlycoprotein)bindpoorlytoAlbuVoid™.Forquantitativestudieswithintheseclassesofproteins,AlbuSorb™PLUSisrecommended.TheComplementsub-proteomeisespeciallyenrichedbyAlbuVoid™PLUS.Thedigestconditionsmaybiastowardsoneormorefunctionalsub-populations,likelyduetoconformationaltransitionsandprotein-proteininteractions(i.e.,FactorBb,Properdin)thatoccuruponactivation.Thisneedsfurtherinvestigation.Thelowabundancesub-proteomeisenriched5+foldwithAlbuVoid™and4+foldwithAlbuSorb™PLUS.ProductSize#SerumPrepsItemNo.AlbuVoid™PLUSKit5preps5,25µlSerumsamplesNP-AVK-05AlbuVoid™PLUSKit10preps10,25µlSerumsamplesNP-AVK-10ClickHereForTheAlbuVoid™PlusProductSheet. 蚂蚁淘(www.ebiomall.com)是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台, 该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁 淘搜索全球供应信息,找到合适的产品后在蚂蚁淘下单,然后蚂蚁淘的海外买手进行跨境采购、 运输到中国口岸,最后由蚂蚁淘国内团队报关运输给客户... 蚂蚁淘所有产品都是自运营的,我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权; 同时客户可以通过订单详情查看到货物从厂方至客户的所有流程, 确保货物的来源; 正规报关,提供13%增值税发票。 蚂蚁淘的运营团队都是有着多年经验的成员,他们熟悉海外采购、仓储物流、报关等环节; 同时通过在线的流程监控,蚂蚁淘的进口速度比传统企业提高了50%以上, 部分产品甚至能做到7-10天到货,即蚂蚁淘的\"时必达”服务。 蚂蚁淘的价格是真实透明的,并且具有很大的价格优势,不需要繁杂的询价比价; 报价单与合同可以直接在线生成或打印;就像在京东购物一样, 您的鼠标点击几 次即完成在蚂蚁淘的采购,订单详情会告诉您所有进程。 蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时, 您可通过我的订单提交您的\"申请售后”, 蚂蚁淘产品顾问会第一时间为您处理, 在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线:4000-520-616。
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